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<p class="MsoNormal"><span style="color:black;mso-fareast-language:JA"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="color:black;mso-fareast-language:JA"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span lang="NL" style="font-size:7.5pt;font-family:"Arial",sans-serif;color:black;mso-fareast-language:NL">Seino A. K. Jongkees, Ph.D.<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:7.5pt;font-family:"Arial",sans-serif;color:black;mso-fareast-language:NL">Associate Professor<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:7.5pt;font-family:"Arial",sans-serif;color:#0089CF;mso-fareast-language:NL">AIMMS Amsterdam Institute of Molecular and Life Sciences</span><span lang="EN-US" style="font-size:7.5pt;font-family:"Arial",sans-serif;color:#5B5B5B;mso-fareast-language:NL"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-GB" style="font-size:12.0pt;font-family:"Times New Roman",serif;color:black;mso-fareast-language:EN-GB"><img width="312" height="63" style="width:3.25in;height:.6583in" id="Picture_x0020_2" src="cid:image002.png@01D95CB6.A5045C70" alt="email_vu_logo_aimms_en.png"></span><span lang="EN-GB" style="font-size:12.0pt;font-family:"Times New Roman",serif;color:black;mso-fareast-language:NL"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:7.5pt;font-family:"Arial",sans-serif;color:#5B5B5B;mso-fareast-language:NL">T +31 (0)6 5722 7168 |
</span><span lang="EN-US" style="font-size:8.5pt;font-family:"Helvetica",sans-serif;color:#4472C4;mso-fareast-language:JA">http://sakjongkees.wixsite.com/website</span><span lang="EN-US" style="font-size:7.5pt;font-family:"Arial",sans-serif;color:#5B5B5B;mso-fareast-language:NL">
| </span><span lang="EN-US" style="font-size:8.5pt;font-family:"Helvetica",sans-serif;color:#4472C4;mso-fareast-language:JA">s.a.k.jongkees@vu.nl</span><span lang="EN-US" style="font-size:7.5pt;font-family:"Arial",sans-serif;color:#5B5B5B;mso-fareast-language:NL"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:7.5pt;font-family:"Arial",sans-serif;color:#5B5B5B;mso-fareast-language:NL">Vrije Universiteit Amsterdam, Chemistry and Pharmaceutical Sciences, O|2 building<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="NL" style="font-size:7.5pt;font-family:"Arial",sans-serif;color:#5B5B5B;mso-fareast-language:NL">O2-03W51, De Boelelaan 1108, 1081 HZ Amsterdam, The Netherlands</span><span lang="NL" style="color:black;mso-fareast-language:EN-GB"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="color:black;mso-fareast-language:JA"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span lang="EN-US" style="mso-fareast-language:JA">From:</span></b><span lang="EN-US" style="mso-fareast-language:JA"> Janssen, A.C. (Elwin) <a.c.janssen@vu.nl>
<br>
<b>Sent:</b> Wednesday, 22 March 2023 12:05 pm<br>
<b>To:</b> Beta Syborch <syborch.beta@vu.nl>; BETA Medicinal Chemistry - Design And Synthesis <mecdes.synth.beta@vu.nl>; _list_jongkees-group.beta-bounces <jongkees-group.beta-bounces@listserver.vu.nl>; Korat, Š. (Špela) <s.korat@amsterdamumc.nl>; Schatte Olivier,
H.A. van der (Anne) <h.vanderschatteolivier@vumc.nl>; Bier, P.M. <p.bier@amsterdamumc.nl>; Moco, S. (Sofia) <s.moco@vu.nl>; Poole, D.A. (David) <d.a.poole@vu.nl>; Niels Sijbrandi <sijbrandi@linxispharmaceuticals.com>; Hamilton, D.J. (David) <d.j.hamilton@vu.nl><br>
<b>Cc:</b> Vernimmen, J. (Jacky) <j.vernimmen@vu.nl><br>
<b>Subject:</b> 500 MHz Spectrometer<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span lang="EN-US">Hello NMR users,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">Some of you might have noticed that the integrals (belonging to a single compound) in
<u><sup>1</sup>H spectra recorded on the 500</u> do not show an optimal relation to another. In the
<sup>1</sup>H spectra the integrals in the higher frequency region (aromatic) are in general between 10-20% lower than in the lower frequency region. Though the spectra still look very good and can still be used to quite an extend for qualitative analyses,
it is a serious issue for quantitative experiments. <o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">Therefore, <u>do not use the 500 for quantitative analyses</u>. You can still use the 300 and, when authorized, the 600 for these types of experiments.<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">Sadly, this is a hardware issue that I cannot fix and Bruker has replaced the involved modules with no effect. I am working on a solution.<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">Please let me know if you have any questions.<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">Best,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">Elwin<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><b><span lang="EN-US" style="font-size:8.0pt;mso-fareast-language:NL">Elwin (A.C.) Janssen<o:p></o:p></span></b></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:8.0pt;mso-fareast-language:NL">NMR Facility Manager|Lecturer
<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:8.0pt;mso-fareast-language:NL">Vrije Universiteit Amsterdam
<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:8.0pt;mso-fareast-language:NL">Department of Chemistry & Pharmaceutical Sciences
<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:8.0pt;color:#0D13FF;mso-fareast-language:NL">AIMMS Amsterdam Institute of Molecular and Life Sciences<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:8.0pt;mso-fareast-language:NL">T +31 (0)20 59 87489|
<a href="mailto:a.c.janssen@vu.nl"><span style="color:blue">a.c.janssen@vu.nl</span></a>|
<a href="https://www.vu.nl/nl/over-de-vu/vu-website/e-mail-disclaimer/index.aspx">
<span style="color:blue">Disclaimer</span></a> <o:p></o:p></span></p>
<p class="MsoNormal"><span lang="NL" style="font-size:8.0pt;mso-fareast-language:NL">MAILING ADDRESS: De Boelelaan 1105, 1081HV Amsterdam<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="NL" style="font-size:8.0pt;mso-fareast-language:NL">VISITING ADDRESS: De Boelelaan 1108, 1081HZ Amsterdam
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